bca protein assay kit (Servicebio Inc)

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Servicebio Inc bca protein assay kit
Bca Protein Assay Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bca+kit/assay+bca+kit+protein/pmc13145395-112-13-18
Average 86 stars, based on 1 article reviews

bca protein assay kit - by Bioz Stars, 2026-11

86/100 stars

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Protein Concentration:

Article Title: Machine learning and multi-dimensional transcriptomics reveal the key molecular network of benzo(a)pyrene/NNK in promoting laryngeal cancer and develop prognostic models.
Article Snippet: Benzo(a)pyrene (BaP) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), as typical environmental carcinogens, are widely present in tobacco smoke and air pollution.. Their combined exposure is an important cause of high incidence of laryngeal cancer.. However, the molecular mechanism of their synergistic carcinogenesis remains unclear.

Article Title: Immunomodulatory effect of borax on murine hepatocellular carcinoma induced by diethylnitrosamine.
Article Snippet: .. Ice-lyse for 30 minutes, centrifuge at 12,000 g (4°C, 15 min), collect supernatant, and measure protein concentration using BCA kit (Servicebio, Cat# G2026). .. Mix protein solution with 5× reducing buffer (Servicebio, Cat# G2075) at a 4:1 ratio, denature at 95°C for 10 minutes, run 10% SDS-PAGE for 60 min, and transfer to PVDF membrane.

Article Title: Esketamine ameliorates postoperative cognitive dysfunction aggravated by preoperative acute sleep deprivation in aged mice via the TLR4/NF-κB pathway
Article Snippet: On ice, hippocampal tissues were cut into small pieces and homogenized using RIPA lysis buffer (G2002-100ML; Servicebio, Wuhan, China) containing a protease inhibitor, followed by lysis for 15 min at 4 °C. .. The samples were centrifuged at 12,000 rpm for 15 min at 4 °C, after which the supernatant was obtained, and a BCA kit (G2026-200 T; Servicebio) was employed to assess the protein concentration. ..

Article Title: Duration-dependent transition from reversible to persistent depressive-like behaviors following chronic circadian misalignment: Involvement of impaired BDNF-TrkB signaling and myelin loss.
Article Snippet: Background: Chronic circadian misalignment (CM) is increasingly implicated in the development of depression.. However, the temporal progression of CM-induced neural dysfunction and the reversibility of associated myelin pathology remain poorly understood.. Purpose: This study aimed to investigate how different durations of CM influence depressive-like behaviors, myelination, and oligodendrocyte (OL) development in mice, with particular emphasis on recovery potential and the involvement of brain-derived neurotrophic factor (BDNF) signaling.

Article Title: Aloe vera polysaccharides facilitate diabetic wound healing by promoting macrophage M 2 polarization through inhibition of NLRP3 inflammasome activation.
Article Snippet: Background: Diabetic wound healing impairment poses a serious threat to patient health, underscoring the urgent need for effective therapies.. Aloe polysaccharides (APs), one of the main bioactive components of Aloe vera, have been reported to exhibit diverse biological activities, including antibacterial, antioxidant, anti-obesity, and antitumor effects.. However, their role in diabetic wound repair remains unclear, limiting its translational potential.

Lysis:

Article Title: Nuciferine inhibits pressure overload-induced cardiac remodeling by activating the SENP1-ACSL4-ferroptosis axis
Article Snippet: .. Based on our previous experimental instructions, We used RIPA lysis buffer (Servicebio, Wuhan, China) for protein extraction and BCA kit (Servicebio, Wuhan, China) for protein quantification [ ]. ..

Protein Extraction:

BIA-KA:

Concentration Assay:

Article Title: Silencing MALAT1 represses pathological progression, inflammation, and vascular smooth muscle cell phenotype switching by regulating the SEMA3C-mediated Smad pathway in intracranial aneurysms
Article Snippet: The 72 h-infected VSMCs were lysed with RIPA buffer (Servicebio, China), which was added with protease and phosphatase inhibitor cocktails (Servicebio, China). .. The concentration of total protein was measured with a BCA kit (Servicebio, China). .. A total of 20 μg of thermally denatured protein was separated on a precast gel and transferred to a nitrocellulose membrane (Whatman, United States).

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